in most segments of the kidney. Accepted:  |  Endocytosis of lipid rafts: an identity crisis. Gabrielli MG, Palatroni P, Vincenzetti S. Histochem J. Hypoxia activates the capacity of tumor-associated carbonic anhydrase IX to acidify extracellular pH. A transport metabolon. Role of carbonic anhydrase IX in human tumor cell growth, survival, and invasion. Human carbonic anhydrase IV: cDNA cloning, sequence comparison, and expression in COS cell membranes. Basolateral carbonic anhydrase IV in the proximal tubule is a glycosylphosphatidylinositol-anchored protein. In non-rodent species (e.g. Carbonic anhydrase expression in kidney and renal cancer: implications for diagnosis and treatment. Carbonic anhydrase XII mRNA encodes a hydratase that is differentially expressed along the rabbit nephron. CAII is expressed as a 29 kDa monomeric protein (259 amino acids) in the cytosol of a wide variety of tissues (e.g. Role of histidine 64 in the catalytic mechanism of human carbonic anhydrase II studied with a site-specific mutant. CAII also associates with NHE1, a ubiquitously expressed glycoprotein that protects cells from intracellular acidification by exchanging one intracellular proton for one extracellular sodium. Role of basolateral carbonic anhydrase in proximal tubular fluid and bicarbonate absorption. Localization of carbonic anhydrase XII to the basolateral membrane of H, CAIX is a 54/58 kDa N-glycosylated single-pass transmembrane protein. CAII physically associates with anion exchangers (AE1-3) via an acidic motif in the C-terminal region of the transporter. The cytoplasmic tail of NBC3 contains two putative CAII binding motifs LDDLM (CAB1) and LQDDDD (CAB2); however only mutation of aspartic acid residues (D. This anion exchanger is expressed in the brush border membrane of proximal tubule epithelial cells, where it may mediate chloride/formate exchange, thereby contributing to NaCl reabsorption. Biol Neonate. 5-RO1-AM-26134/AM/NIADDK NIH HHS/United States. Lowered oxygen tension induces expression of the hypoxia marker MN/carbonic anhydrase IX in the absence of hypoxia-inducible factor 1a stabilization: a role for phosphatidylinositol 3′-kinase. Functional consequences of engineering the hydrophobic pocket of carbonic anhydrase II. 2020 Dec;9(2):333-347. doi: 10.1007/s40119-020-00194-3. Proton transfer roles of lysine 64 and glutamic acid 64 replacing histidine 64 in the active site of human carbonic anhydrase II. CAs IX and XII are tumor-associated antigens whose expression is induced by hypoxia. Membrane-bound carbonic anhydrase IV is expressed in the luminal plasma membrane of the human gallbladder epithelium. Targeting of transmembrane and GPI-anchored forms of N-CAM to opposite domains of a polarized epithelial cell. CAII and CAIV predominate in human and rabbit kidneys; in rodent kidneys, CAXII, and CAXIV are also present. Significant advances over the past decade have been made in characterizing the nature of renal carbonic anhydrase.  |  Gastric hyperplasia in mice with targeted disruption of the carbonic anhydrase gene Car9. Purification and properties. 2013 Apr 3;4:59. doi: 10.3389/fphys.2013.00059. CAIV is unique among CA isoforms, because it is tethered to the plasma membrane via a GPI anchor. The catalytic mechanism of carbonic anhydrases: implications of rate-limited protolysis of water. Carbonic anhydrase II binds to the carboxyl terminus of human band 3, the erythrocyte Cl. Glycosyl-phosphatidylinositol-anchored membrane proteins. Most if not all BT family members contain a consensus CAII binding motif consisting of a hydrophobic residue followed by at least two acid residues within the next four amino acids. Carbonic anhydrase histochemistry in rabbit and mouse kidneys. 1990 Nov;22(11):579-87. doi: 10.1007/BF01072939. Renal membrane-bound carbonic anhydrase. Wandernoth PM, Mannowetz N, Szczyrba J, Grannemann L, Wolf A, Becker HM, Sly WS, Wennemuth G. J Biol Chem. Carbonic anhydrase IV expression in rat and human gastrointestinal tract regional, cellular, and subcellular localization. Published by Elsevier Inc. IGF-1 vs insulin: Respective roles in modulating sodium transport via the PI-3 kinase/Sgk1 pathway in a cortical collecting duct cell line, OVERVIEW OF CARBONIC ANHYDRASE FUNCTION IN REGULATION OF ACID/BASE HOMEOSTASIS IN CELLS OF THE PROXIMAL AND DISTAL NEPHRON AND RCC, We use cookies to help provide and enhance our service and tailor content and ads. This site needs JavaScript to work properly. Carbonic anhydrase is a zinc metalloenzyme widely distributed throughout the tissues of the body. Carbonic anhydrase in the metanephrogenic zone of the human fetal kidney. CAXII is a one-pass transmembrane protein expressing an extracellular CA domain with a unique short extracellular segment and a hydrophilic cytoplasmic tail, and one pair of conserved cysteine residues that putatively form one intramolecular disulfide bond. In mice, human, and rabbit species, CAXIV is abundantly expressed in heart, skeletal muscle, liver, and in mice and humans abundant expression was also observed in brain. They are therefore classified as metalloenzymes. Please enable it to take advantage of the complete set of features! Expression of hypoxia-inducible cell-surface transmembrane carbonic anhydrases in human cancer. 1982 Nov;204(3):185-97. doi: 10.1002/ar.1092040303. In addition, a membrane-bound form constituting only about 2--5% of the renal activity has been found in the brush border and basolateral fractions of kidney homogenates. Two distinct isozymes of carbonic anhydrase have now been identified in the mammalian kidney. 2006, Received: HHS bone, brain, eye, stomach, intestine, liver, pancreas, kidney, red blood cells, salivary glands, and uterus). 2014;75:181-98. doi: 10.1007/978-94-007-7359-2_10. Expression of carbonic anhydrase IX in mouse tissues. Down-regulation of transmembrane carbonic anhydrases in renal cell carcinoma cell lines by wild-type von Hippel–Lindau transgenes. Carbonic anhydrase IV: Role of removal of C-terminal domain in glycosylphosphatidylinositol anchoring and realization of enzyme activity. By continuing you agree to the, The role of carbonic anhydrases in renal physiology, View Large NIH Nucleotide sequence of a cDNA encoding rat brain carbonic anhydrase II and its deduced amino acid sequence. As the renin–angiotensin system regulates pancreatic bicarbonate secretion via a protein kinase C (PKC)-dependent mechanism. October 10, September 29, Localization and activity of renal carbonic anhydrase (CA) in CA-II deficient mice. Reduced activity of some CA isoforms (CAIII), as well as species differences in CA isoform activity, provides additional insights into the function of other amino-acid residues within the CA catalytic domain.


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